Correlated Cryogenic Photoactivated Localization Microscopy and Cryo-Electron Tomography

Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11.

Abstract

Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization microscopy)-CET method for localizing objects within cryo-tomograms to beyond the diffraction limit of the light microscope. Using cryo-PALM-CET, we identified multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Secretion Systems*
  • Cryoelectron Microscopy / methods*
  • Electron Microscope Tomography / methods*
  • Imaging, Three-Dimensional / methods
  • Myxococcus xanthus / chemistry
  • Myxococcus xanthus / ultrastructure*

Substances

  • Bacterial Secretion Systems