Whole genome mapping of the first reported case of KPC-2-positive Klebsiella pneumoniae ST258 in Nebraska

Randal C Fowler; Caitlyn R Scharn; Mir A Ali; Stephen J Cavalieri; Richard V Goering; Nancy D Hanson

doi:10.1016/j.diagmicrobio.2014.03.023

Whole genome mapping of the first reported case of KPC-2-positive Klebsiella pneumoniae ST258 in Nebraska

Diagn Microbiol Infect Dis. 2014 Jul;79(3):384-6. doi: 10.1016/j.diagmicrobio.2014.03.023. Epub 2014 Apr 12.

Authors

Randal C Fowler¹, Caitlyn R Scharn¹, Mir A Ali¹, Stephen J Cavalieri¹, Richard V Goering¹, Nancy D Hanson²

Affiliations

¹ Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178.
² Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Electronic address: ndhanson@creighton.edu.

PMID: 24813687
DOI: 10.1016/j.diagmicrobio.2014.03.023

Abstract

Three ertapenem-resistant Klebsiella pneumoniae carrying bla(KPC-2) were isolated from a single patient in Nebraska over a span of 5 months. A comparative analysis of the genetic relatedness of these isolates was investigated using pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome mapping.

Keywords: MLST; PFGE; Whole genome mapping; bla(KPC-2).

Publication types

Case Reports

MeSH terms

Anti-Bacterial Agents / pharmacology
Chromosome Mapping*
Electrophoresis, Gel, Pulsed-Field
Genotype
Humans
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / classification
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Male
Microbial Sensitivity Tests
Middle Aged
Multilocus Sequence Typing
Nebraska
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
beta-lactamase KPC-2
beta-Lactamases