Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages

Int Immunopharmacol. 2014 Jul;21(1):186-92. doi: 10.1016/j.intimp.2014.04.021. Epub 2014 May 9.


The TNF-α expression-inhibitory effect of lactic acid bacteria (LAB) isolated from kimchi were measured in lipopolysaccharide (LPS)-stimulated peritoneal macrophages. Among the LAB evaluated, Lactobacillus plantarum CLP-0611 inhibited the IL-1β and IL-6 expression, as well as the NF-κB and AP1 activation in LPS-stimulated peritoneal macrophages. Therefore, we investigated its inhibitory effect on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. TNBS significantly induced colon shortening, as well as myeloperoxidase activity and macroscopic score. Oral administration of CLP-0611 significantly reduced TNBS-induced body weight loss, colon shortening, myeloperoxidase activity, IRAK-1 phosphorylation, NF-κB and MAP kinase (p38, ERK, JNK) activation, and iNOS and COX-2 expression. CLP-0611 also inhibited TNBS-induced expression of TNF-α, IL-1β, and IL-6. However, IL-10 expression was induced. CLP-0611 also induced the production of M2 macrophage markers (IL-10, arginase I and CD206). Based on these findings, CLP-0611 inhibits TLR-4-linked NF-κB and MAPK signaling pathways and polarizes M1 to M2-like macrophages, thus ameliorating colitis.

Keywords: Colitis; Lactobacillus plantarum CLP-0611; Macrophage; Polarization; TNF-α.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Colitis / chemically induced
  • Colitis / immunology*
  • Colitis / microbiology
  • Colitis / therapy
  • Colon / pathology*
  • Cytokines / genetics
  • Cytokines / metabolism
  • Interleukin-1 Receptor-Associated Kinases / metabolism
  • Lactobacillus plantarum / immunology*
  • Lipopolysaccharides / immunology
  • MAP Kinase Signaling System / immunology
  • Macrophages, Peritoneal / immunology*
  • Macrophages, Peritoneal / microbiology
  • Male
  • Mice
  • Mice, Inbred ICR
  • NF-kappa B / metabolism
  • Peroxidase / metabolism
  • Probiotics / administration & dosage*
  • Transcription Factor AP-1 / metabolism
  • Trinitrobenzenesulfonic Acid / administration & dosage


  • Cytokines
  • Lipopolysaccharides
  • NF-kappa B
  • Transcription Factor AP-1
  • Trinitrobenzenesulfonic Acid
  • Peroxidase
  • Interleukin-1 Receptor-Associated Kinases