An integrated map of HIV-human protein complexes that facilitate viral infection

PLoS One. 2014 May 9;9(5):e96687. doi: 10.1371/journal.pone.0096687. eCollection 2014.


Recent proteomic and genetic studies have aimed to identify a complete network of interactions between HIV and human proteins and genes. This HIV-human interaction network provides invaluable information as to how HIV exploits the host machinery and can be used as a starting point for further functional analyses. We integrated this network with complementary datasets of protein function and interaction to nominate human protein complexes with likely roles in viral infection. Based on our approach we identified a global map of 40 HIV-human protein complexes with putative roles in HIV infection, some of which are involved in DNA replication and repair, transcription, translation, and cytoskeletal regulation. Targeted RNAi screens were used to validate several proteins and complexes for functional impact on viral infection. Thus, our HIV-human protein complex map provides a significant resource of potential HIV-host interactions for further study.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • HEK293 Cells
  • HIV Infections / genetics
  • HIV Infections / metabolism*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / metabolism*
  • HIV-1 / physiology
  • Host-Pathogen Interactions
  • Human Immunodeficiency Virus Proteins / genetics
  • Human Immunodeficiency Virus Proteins / metabolism*
  • Humans
  • Jurkat Cells
  • Protein Binding
  • Protein Interaction Mapping / methods
  • Protein Interaction Maps*
  • Proteomics / methods
  • RNA Interference
  • Signal Transduction


  • Human Immunodeficiency Virus Proteins