Alpha-B-crystallin expression in human laryngeal squamous cell carcinoma tissues

Head Neck. 2015 Sep;37(9):1344-8. doi: 10.1002/hed.23746. Epub 2014 Jul 19.


Background: Laryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. The recent studies suggested that αB-crystallin might play an important role in tumorigenesis. The purpose of this study was to investigate the αB-crystallin expression level in metastatic and nonmetastatic laryngeal SCC tissues and to determine its prognostic significance.

Methods: Alpha-B-crystallin expression status in metastatic, nonmetastatic laryngeal SCC, and normal tissue samples was investigated by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: We demonstrated that the expression of αB-crystallin was significantly upregulated in laryngeal SCC tumor tissue samples in comparison with the corresponding normal tissues (p < .001), although no significant association has been found between αB-crystallin expression and either the metastatic potential or the T classification of the specimens.

Conclusion: Although expression of αB-crystallin is not statistically correlated with neck metastases, we demonstrated that αB-crystallin is significantly overexpressed in laryngeal cancer.

Keywords: laryngeal squamous cell carcinoma; lymph node; metastasis; quantitative real-time polymerase chain reaction (qRT-PCR); αB-crystallin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biopsy, Needle
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / mortality
  • Carcinoma, Squamous Cell / secondary
  • Carcinoma, Squamous Cell / surgery
  • Case-Control Studies
  • Disease-Free Survival
  • Gene Expression Regulation, Neoplastic
  • Head and Neck Neoplasms / genetics*
  • Head and Neck Neoplasms / mortality
  • Head and Neck Neoplasms / secondary
  • Head and Neck Neoplasms / surgery
  • Humans
  • Immunohistochemistry
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / mortality
  • Laryngeal Neoplasms / pathology
  • Laryngeal Neoplasms / surgery
  • Lymph Nodes / pathology*
  • Lymph Nodes / surgery
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Neoplasm Invasiveness / pathology
  • Neoplasm Staging
  • Prognosis
  • Real-Time Polymerase Chain Reaction / methods
  • Retrospective Studies
  • Squamous Cell Carcinoma of Head and Neck
  • Survival Analysis
  • alpha-Crystallin B Chain / genetics*


  • CRYAB protein, human
  • alpha-Crystallin B Chain