Plate-based assay for swimming motility in Pseudomonas aeruginosa

Methods Mol Biol. 2014;1149:59-65. doi: 10.1007/978-1-4939-0473-0_7.


Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Assay / methods*
  • Movement
  • Pseudomonas aeruginosa / physiology*
  • Quorum Sensing