Abstract
Antimicrobial peptides (AMPs) are garnering attention as possible alternatives to antibiotics. Here, we describe the antimicrobial properties of epinecidin-1 against a multidrug-resistant clinical isolate of P. aeruginosa (P. aeruginosa R) and a P. aeruginosa strain from ATCC (P. aeruginosa ATCC 19660) in vivo. The MICs of epinecidin-1 against P. aeruginosa R and P. aeruginosa ATCC 19660 were determined and compared with those of imipenem. Epinecidin-1 was found to be highly effective at combating peritonitis infection caused by P. aeruginosa R or P. aeruginosa ATCC 19660 in mouse models, without inducing adverse behavioral effects or liver or kidney toxicity. Taken together, our results indicate that epinecidin-1 enhances the rate of survival of mice infected with the bacterial pathogen P. aeruginosa through both antimicrobial and immunomodulatory effects.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / pharmacology
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Antimicrobial Cationic Peptides / chemical synthesis
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Antimicrobial Cationic Peptides / pharmacology*
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Behavior, Animal / drug effects
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Disease Models, Animal
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Drug Resistance, Multiple, Bacterial
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Fish Proteins / chemical synthesis
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Fish Proteins / pharmacology*
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Humans
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Imipenem / pharmacology
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Immunologic Factors / chemical synthesis
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Immunologic Factors / pharmacology*
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Male
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Mice
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Mice, Inbred C57BL
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Pseudomonas Infections / drug therapy*
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Pseudomonas Infections / immunology
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Pseudomonas Infections / microbiology
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Pseudomonas Infections / mortality
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / growth & development
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Pseudomonas aeruginosa / pathogenicity*
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Sepsis / drug therapy*
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Sepsis / immunology
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Sepsis / microbiology
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Sepsis / mortality
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Survival Analysis
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Toxicity Tests, Acute
Substances
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Anti-Bacterial Agents
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Antimicrobial Cationic Peptides
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Fish Proteins
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Immunologic Factors
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epinecidin-1, Epinephelus coioides
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Imipenem