Boseongicola Aestuarii Gen. Nov., Sp. Nov., Isolated From a Tidal Flat Sediment

Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2618-2624. doi: 10.1099/ijs.0.061960-0. Epub 2014 May 13.

Abstract

A Gram-stain-negative, aerobic, non-motile and pleomorphic (coccoid, ovoid or rod-shaped) bacterial strain, BS-W15(T), isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-W15(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of approximately 2.0% (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W15(T) joined the cluster comprising the type strains of Profundibacterium mesophilum, Hwanghaeicola aestuarii, M. pelagius and M. salinus, showing 93.5-96.4% sequence similarities. Strain BS-W15(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c as the predominant fatty acid. The polar lipid profile of strain BS-W15(T) contained phosphatidylcholine and phosphatidylglycerol as major components, differentiating it from those of the type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. The DNA G+C content of strain BS-W15(T) was 58.7 mol%. The differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrate that strain BS-W15(T) is distinct from type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. On the basis of the data presented, strain BS-W15(T) is considered to represent a novel genus and species, for which the name Boseongicola aestuarii gen. nov., sp. nov. is proposed. The type strain is BS-W15(T) ( = KCTC 32576(T) = CECT 8489(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Geologic Sediments / microbiology*
  • Molecular Sequence Data
  • Phosphatidylcholines / chemistry
  • Phosphatidylglycerols / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Seawater / microbiology
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylcholines
  • Phosphatidylglycerols
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • Ubiquinone Q2

Associated data

  • GENBANK/KF977837