Abstract
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2 encoding the sarcoplasmic reticulum Ca2+ pump in slow skeletal and cardiac muscle. This alternatively spliced transcript was shown to be mainly represented in non-muscle tissues. These antibodies have enabled us to show the presence of the unique C-terminus of this type of Ca2+ pump, as predicted from the cDNA sequence, in the endoplasmic reticulum of vascular and gastric smooth muscle, liver and kidney.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies*
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Aorta / enzymology
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Calcium-Transporting ATPases / genetics*
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Calcium-Transporting ATPases / immunology
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Cattle
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Cell Membrane / enzymology
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Endoplasmic Reticulum / enzymology*
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Epitopes / analysis*
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Genes
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Isoenzymes / genetics*
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Isoenzymes / immunology
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Microsomes / enzymology
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Molecular Sequence Data
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Muscle, Smooth / enzymology*
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Muscle, Smooth, Vascular / enzymology
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Muscles / enzymology*
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Oligopeptides / chemical synthesis
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Peptide Fragments / analysis
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RNA Splicing
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Rabbits / immunology
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Stomach / enzymology
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Swine
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Transcription, Genetic
Substances
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Antibodies
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Epitopes
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Isoenzymes
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Oligopeptides
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Peptide Fragments
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Calcium-Transporting ATPases