A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells

Biotechnol Prog. Jul-Aug 2014;30(4):952-9. doi: 10.1002/btpr.1928. Epub 2014 May 28.


We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2, 3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy.

Keywords: cell imaging; conjugation; fluorescence microscopy; folic acid.

MeSH terms

  • Diagnostic Imaging
  • Fluorescent Dyes / chemistry*
  • Folate Receptor 1 / biosynthesis
  • Folate Receptor 1 / isolation & purification*
  • Folic Acid / analogs & derivatives*
  • Folic Acid / chemistry*
  • Gene Expression Regulation*
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Microscopy, Fluorescence
  • Nanoparticles / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Triazoles / chemistry*


  • Fluorescent Dyes
  • Folate Receptor 1
  • Triazoles
  • folic aid-6-(4,7-bis(2,3-dihydrothieno(3,4-b)(1,4)dioxin-5-yl)-2H-benzo(d)(1,2, 3)triazol-2-yl)hexan-1-amine
  • Folic Acid