Genomic analyses of pneumococci from children with sickle cell disease expose host-specific bacterial adaptations and deficits in current interventions

Cell Host Microbe. 2014 May 14;15(5):587-599. doi: 10.1016/j.chom.2014.04.005.


Sickle cell disease (SCD) patients are at high risk of contracting pneumococcal infection. To address this risk, they receive pneumococcal vaccines, and antibiotic prophylaxis and treatment. To assess the impact of SCD and these interventions on pneumococcal genetic architecture, we examined the genomes of more than 300 pneumococcal isolates from SCD patients over 20 years. Modern SCD strains retained invasive capacity but shifted away from the serotypes used in vaccines. These strains had specific genetic changes related to antibiotic resistance, capsule biosynthesis, metabolism, and metal transport. A murine SCD model coupled with Tn-seq mutagenesis identified 60 noncapsular pneumococcal genes under differential selective pressure in SCD, which correlated with aspects of SCD pathophysiology. Further, virulence determinants in the SCD context were distinct from the general population, and protective capacity of potential antigens was lost over time in SCD. This highlights the importance of understanding bacterial pathogenesis in the context of high-risk individuals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Adolescent
  • Anemia, Sickle Cell / complications*
  • Bacterial Proteins / genetics*
  • Child
  • Child, Preschool
  • Female
  • Genomics
  • Host-Pathogen Interactions*
  • Humans
  • Infant
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Pneumococcal Infections / etiology
  • Pneumococcal Infections / microbiology*
  • Pneumococcal Infections / prevention & control
  • Pneumococcal Vaccines / administration & dosage
  • Prospective Studies
  • Streptococcus pneumoniae / classification
  • Streptococcus pneumoniae / genetics*
  • Streptococcus pneumoniae / isolation & purification
  • Streptococcus pneumoniae / physiology


  • Bacterial Proteins
  • Pneumococcal Vaccines