Pseudomonas syringae CC1557: a highly virulent strain with an unusually small type III effector repertoire that includes a novel effector

Mol Plant Microbe Interact. 2014 Sep;27(9):923-32. doi: 10.1094/MPMI-11-13-0354-R.

Abstract

Both type III effector proteins and nonribosomal peptide toxins play important roles for Pseudomonas syringae pathogenicity in host plants, but whether and how these pathways interact to promote infection remains unclear. Genomic evidence from one clade of P. syringae suggests a tradeoff between the total number of type III effector proteins and presence of syringomycin, syringopeptin, and syringolin A toxins. Here, we report the complete genome sequence from P. syringae CC1557, which contains the lowest number of known type III effectors to date and has also acquired genes similar to sequences encoding syringomycin pathways from other strains. We demonstrate that this strain is pathogenic on Nicotiana benthamiana and that both the type III secretion system and a new type III effector, hopBJ1, contribute to pathogenicity. We further demonstrate that activity of HopBJ1 is dependent on residues structurally similar to the catalytic site of Escherichia coli CNF1 toxin. Taken together, our results provide additional support for a negative correlation between type III effector repertoires and the potential to produce syringomycin-like toxins while also highlighting how genomic synteny and bioinformatics can be used to identify and characterize novel virulence proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Gene Expression Regulation, Bacterial
  • Genome, Bacterial / genetics*
  • Genomics
  • Host-Pathogen Interactions*
  • Models, Molecular
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / microbiology*
  • Plant Leaves
  • Pseudomonas syringae / genetics*
  • Pseudomonas syringae / pathogenicity
  • Sequence Analysis, DNA
  • Species Specificity
  • Tobacco / microbiology*
  • Virulence

Substances

  • Bacterial Proteins
  • syringomycin

Associated data

  • GENBANK/AVEG00000000
  • GENBANK/AVEH00000000
  • GENBANK/AVEM00000000