Roles of chromatin remodelers in maintenance mechanisms of multipotency of mouse trunk neural crest cells in the formation of neural crest-derived stem cells

Kyohei Fujita; Ryuhei Ogawa; Syunsaku Kawawaki; Kazuo Ito

doi:10.1016/j.mod.2014.05.001

Roles of chromatin remodelers in maintenance mechanisms of multipotency of mouse trunk neural crest cells in the formation of neural crest-derived stem cells

Mech Dev. 2014 Aug:133:126-45. doi: 10.1016/j.mod.2014.05.001. Epub 2014 May 15.

Authors

Kyohei Fujita¹, Ryuhei Ogawa¹, Syunsaku Kawawaki¹, Kazuo Ito²

Affiliations

¹ Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
² Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. Electronic address: itokazuo@bio.sci.osaka-u.ac.jp.

PMID: 24836203
DOI: 10.1016/j.mod.2014.05.001

Abstract

We analyzed roles of two chromatin remodelers, Chromodomain Helicase DNA-binding protein 7 (CHD7) and SWItch/Sucrose NonFermentable-B (SWI/SNF-B), and Bone Morphogenetic Protein (BMP)/Wnt signaling in the maintenance of the multipotency of mouse trunk neural crest cells, leading to the formation of mouse neural crest-derived stem cells (mouse NCSCs). CHD7 was expressed in the undifferentiated neural crest cells and in the dorsal root ganglia (DRG) and sciatic nerve, typical tissues containing NCSCs. BMP/Wnt signaling stimulated the expression of CHD7 and participated in maintaining the multipotency of neural crest cells. Furthermore, the promotion of CHD7 expression maintained the multipotency of these cells. The inhibition of CHD7 and SWI/SNF-B expression significantly suppressed the maintenance of the multipotency of these cells. In addition, BMP/Wnt treatment promoted CHD7 expression and caused the increase of the percentage of multipotent cells in DRG. Thus, the present data suggest that the chromatin remodelers as well as BMP/Wnt signaling play essential roles in the maintenance of the multipotency of mouse trunk neural crest cells and in the formation of mouse NCSCs.

Keywords: BMP/Wnt signaling; Chromatin remodeler; Mouse; Neural crest-derived stem cells.

MeSH terms

Animals
Apoptosis
Bone Morphogenetic Proteins / metabolism
Cell Differentiation
Cell Proliferation
Cells, Cultured
Chromatin Assembly and Disassembly*
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
DNA-Binding Proteins / antagonists & inhibitors
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism
Ganglia, Spinal / cytology
Ganglia, Spinal / embryology
Ganglia, Spinal / metabolism
Gene Expression Regulation, Developmental
Mice
Multipotent Stem Cells / cytology
Multipotent Stem Cells / metabolism
Neural Crest / cytology*
Neural Crest / metabolism
Neural Stem Cells / cytology*
Neural Stem Cells / metabolism
RNA, Small Interfering / genetics
Receptors, Nerve Growth Factor / genetics
Receptors, Nerve Growth Factor / metabolism
SOXE Transcription Factors / genetics
SOXE Transcription Factors / metabolism
Sciatic Nerve / cytology
Sciatic Nerve / embryology
Sciatic Nerve / metabolism
Signal Transduction
Transcription Factors / genetics
Transcription Factors / metabolism
Wnt Signaling Pathway

Substances

Bone Morphogenetic Proteins
Chd7 protein, mouse
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
RNA, Small Interfering
Receptors, Nerve Growth Factor
SOXE Transcription Factors
SWI-SNF-B chromatin-remodeling complex
Sox10 protein, mouse
Ngfr protein, mouse
Transcription Factors