Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy

Nat Methods. 2014 Jul;11(7):727-730. doi: 10.1038/nmeth.2964. Epub 2014 May 18.


High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Calcium / metabolism*
  • Calcium Signaling
  • Imaging, Three-Dimensional / methods*
  • Larva / ultrastructure
  • Microscopy / methods*
  • Microscopy, Fluorescence / methods
  • Neurons / physiology*
  • Zebrafish


  • Calcium