Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns

Richard S Song; Jeanne M Carroll; Lisette Acevedo; Dongmei Wu; Yang Liu; Evan Y Snyder

doi:10.1002/9780470151808.sc01c16s29

Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns

Curr Protoc Stem Cell Biol. 2014 May 16:29:1C.16.1-13. doi: 10.1002/9780470151808.sc01c16s29.

Authors

Richard S Song¹, Jeanne M Carroll, Lisette Acevedo, Dongmei Wu, Yang Liu, Evan Y Snyder

Affiliation

¹ Department of Pediatrics, University of California at San Diego and Rady Children's Hospital, San Diego, California.

PMID: 24838913
DOI: 10.1002/9780470151808.sc01c16s29

Abstract

The umbilical cord is tissue that is normally discarded after the delivery of the infant, but it has been shown to be a rich source of stem cells from the cord blood, Wharton's jelly, and umbilical endothelial cells. Patient-specific human induced pluripotent stem cells (hiPSCs) reprogrammed from patient specific human umbilical vein endothelial cells in the neonatal intensive care unit (NICU) population (specifically, premature neonates) have not been shown in the literature. This unit describes a protocol for the generation and expansion of hiPSCs originating from umbilical cords collected from patients in the NICU.

Keywords: HUVEC; iPS; reprogramming; umbilical cord.

Publication types

Review

MeSH terms

Cell Differentiation*
Cellular Reprogramming Techniques / methods*
Human Umbilical Vein Endothelial Cells* / cytology
Human Umbilical Vein Endothelial Cells* / metabolism
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Infant, Newborn
Umbilical Cord* / cytology
Umbilical Cord* / metabolism