Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, (406), 41-64
eCollection

Taxonomy of the Colocasiomyia Gigantea Species Group (Diptera, Drosophilidae), With Descriptions of Four New Species From Yunnan, China

Affiliations

Taxonomy of the Colocasiomyia Gigantea Species Group (Diptera, Drosophilidae), With Descriptions of Four New Species From Yunnan, China

Nan-Nan Li et al. Zookeys.

Abstract

Species of the genus Colocasiomyia de Meijere feed/breed on inflorescences/infructescences of the plants from the families Araceae, Arecaceae and Magnoliaceae. Although most of them utilize plants from the subfamily Aroideae of Araceae, three species of the recently established C. gigantea species group make use of plants of the subfamily Monsteroideae. We describe four new species of the gigantea group found from Yunnan, China: Colocasiomyia longifilamentata Li & Gao, sp. n., C. longivalva Li & Gao, sp. n., C. hailini Li & Gao, sp. n., and C. yini Li & Gao, sp. n. The species delimitation is proved in virtue of not only morphology but also DNA barcodes, i.e., sequences of the partial mitochondrial COI (cytochrome c oxidase subunit I) gene. Some nucleotide sites with fixed status in the alignment of the COI sequences (658 sites in length) are used as "pure" molecular diagnostic characters to delineate species in the gigantea group.

Keywords: Adaptation; Rhaphidophora clade; aroid; character-based barcoding; cohabitation; genetic distance; integrated taxonomy.

Figures

Figure 1.
Figure 1.
Diagnostic nucleotide sites in the alignment of COI sequences of the Colocasiomyia gigantea group. Numbers at the top show the positions of the sites in the COI alignment (658-bp in length). Shaded sites are diagnostic for each species. Hyphens (-) indicate end missing data.
Figures 2–22.
Figures 2–22.
SEM photographs showing leg and oviscapt fine structures in the Colocasiomyia gigantea species group. Foreleg tarsomeres I and II (2–8), pegs on foreleg tarsomere II (9–15) and warts on basal part of lateral lobe or basal membrane of oviscapt (16–22) of Colocasiomyia gigantea (2, 9, 16), Colocasiomyia scindapsae (3, 10, 17), Colocasiomyia rhaphidophorae (4, 11, 18), Colocasiomyia longifilamentata sp. n. (5, 12, 19), Colocasiomyia longivalva sp. n. (6, 13, 20), Colocasiomyia hailini sp. n. (7, 14, 21) and Colocasiomyia yini sp. n. (8, 15, 22). Scale line = 0.1 mm in 2–8, 0.05 mm in 9–15. Figures 16–22 are in the same magnification, with the width corresponding to 30 µm.
Figures 23–28.
Figures 23–28.
Colocasiomyia longifilamentata Li & Gao, sp. n. Adult male and female (paratypes) from Baihualing, Yunnan, China: 23 Cibarium and clypeus (dorsal view) 24 periphallic organs (posterolateral view) 25 apical part of surstylus 26 phallic organs (lateral view) 27 hypandrium and parameres (ventral view) 28 oviscapt (lateral view). Abbreviations: aed = aedeagus, aed a = aedeagal apodeme, a s = anterior sensilla, cerc = cercus, cly = clypeus, epand = epandrium, epand a = epandrial apodeme, hypd = hypandrium, m s = medial sensilla, ps = posterior sensilla, pm = paramere, s c = sensilla campaniformia, sur = surstylus. Scale lines = 0.1 mm.
Figures 29–36.
Figures 29–36.
Colocasiomyia longivalva Li & Gao, sp. n. Adult male and female (paratypes) from Baihualing, Yunnan, China: 29 Cibarium and clypeus (dorsal view) 30 periphallic organs (posterolateral view) 31 periphallic organs (ventral view) 32 apical part of epandrial ventral lobe (ventral view) 33 surstylus (ventral view) 34 phallic organs (lateral view) 35 hypandrium and parameres (ventral view) 36 oviscapt (lateral view). Scale lines = 0.1 mm.
Figures 37–50.
Figures 37–50.
Colocasiomyia hailini Li & Gao, sp. n. (37–43) and Colocasiomyia yini Li & Gao, sp. n. (44–50). Adult males and females (paratypes) from Baihualing, Yunnan, China: 37, 44 Cibarium and clypeus (dorsal view) 38, 45 periphallic organs (posterolateral view) 39, 46 surstylus 40, 47 phallic organs (lateral view) 41, 48 apical part of aedeagus (ventral view) 42, 49 hypandrium and parameres (ventral view) 43, 50 oviscapt (lateral view). Scale lines = 0.1 mm.

Similar articles

See all similar articles

Cited by 4 articles

References

    1. Carson HL, Okada T. (1980) Drosophilidae associated with flowers in Papua New Guinea. I. Colocasia esculenta. Kontyû 48: 15-29
    1. Cusimano N, Bogner J, Maya SJ, Boyce PC, Wong SY, Hesse M, Hetterscheid WLA, Keating R, French JC. (2011) Relationships within the Araceae: comparison of morphological patterns with molecular phylogenies. American Journal of Botany 98: 654-668. doi: 10.3732/ajb.1000158 - DOI - PubMed
    1. DeSalle R, Egan MG, Siddall M. (2005) The unholy trinity: taxonomy, species delimitation and DNA barcoding. Philosophical Transactions of the Royal Society B 360: 1905-1916. doi: 10.1098/rstb.2005.1722 - DOI - PMC - PubMed
    1. Fartyal RS, Gao JJ, Toda MJ, Hu YG, Takano KT, Suwito A, Katoh T, Takigahira T, Yin JT. (2013) Colocasiomyia (Diptera: Drosophilidae) revised phylogenetically, with a new species group having peculiar lifecycles on monsteroid (Araceae) host plants. Systematic Entomology 38: 763-782. doi: 10.1111/syen.12027 - DOI
    1. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294-299 - PubMed

LinkOut - more resources

Feedback