Nocardioides nanhaiensis sp. nov., an actinobacterium isolated from a marine sediment sample

Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2718-2722. doi: 10.1099/ijs.0.062851-0. Epub 2014 May 20.


A rod- to coccus-shaped, non-spore-forming actinobacterium, strain YIM M13091(T), was isolated from a marine sediment sample collected from the South China Sea and examined by a polyphasic approach to clarify its taxonomic position. This Gram-staining-positive, aerobic actinobacterium did not produce substrate mycelium and aerial hyphae, and no diffusible pigments were produced on the media tested. The optimum growth occurred at 30 °C, 1% (w/v) NaCl and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Nocardioides, with low levels (≤96.2%) of sequence similarity with respect to Nocardioides kribbensis KSL-2(T) and other members of the genus Nocardioides. Whole-organism hydrolysates of the strain contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H4), with MK-8 in a minor amount. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, hydroxyphosphatidylinositol and phosphatidylcholine, were the main polar lipids detected, while iso-C(16 : 0) and C(18 : 1)ω9c were the major fatty acids. The G+C content of the genomic DNA was 68.5 mol%. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a member of the genus Nocardioides, and the name Nocardioides nanhaiensis sp. nov. (Type strain YIM M13091(T) = JCM 18127(T) = CCTCC AA 2011020(T)) is proposed for the novel species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Geologic Sediments / microbiology*
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • vitamin MK 8
  • Diaminopimelic Acid

Associated data

  • GENBANK/KJ013499