Protective effect of administration of Withania somifera against bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats

Ren Fail. 2014 Aug;36(7):1095-103. doi: 10.3109/0886022X.2014.918812. Epub 2014 May 21.


Background: The present study was conducted to elucidate the protective role of Withania somnifera against bromobenzene induced nephrotoxicity and mitochondrial dysfunction in rats.

Methods: In this study, Wistar albino rats of either sex were divided into six groups consisting of six animals each. The first one was control, those in group II received bromobenzene (10 mmol/kg, intragastric intubation) once, but group III and IV animals received W. somnifera (250 and 500 mg/kg, orally), respectively for 8 days followed by bromobenzene once on the 8th day and silymarin (100 mg/kg, orally) was given for 8 days to group V animals and then bromobenzene on the last day. Group VI animals received only W. somnifera (500 mg/kg) for 8 days.

Results: The levels of renal lipid peroxidation, lysosomal enzymes and glycoprotein were increased significantly (p<0.05) in the bromobenzene alone treated rats and antioxidant status and mitochondrial enzymes were found to be decreased, when compared to the control group. The levels of kidney functional markers (urea, uric acid and creatinine) were also found to be abnormal in serum of bromobenzene alone treated rats. On the other hand, administration of W. somnifera (250 and 500 mg/kg) along with bromobenzene offered a significant dose-dependent protection to the biochemical alterations as observed in the bromobenzene alone treated rats, which was also evidenced by histopathology.

Conclusion: Thus, the oral administration of W. somnifera significantly protected against the bromobenzene induced nephrotoxicity and renal mitochondrial dysfunction in rats.

Keywords: Antioxidant; glutathione; lipid peroxidation; nephroprotective.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Bromobenzenes
  • Drug Evaluation, Preclinical
  • Kidney / drug effects
  • Kidney / enzymology
  • Kidney / pathology
  • Lysosomes / enzymology
  • Mitochondria / drug effects
  • Mitochondria / enzymology
  • Oxidative Stress / drug effects*
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Random Allocation
  • Rats, Wistar
  • Renal Insufficiency / chemically induced
  • Renal Insufficiency / enzymology
  • Renal Insufficiency / pathology
  • Renal Insufficiency / prevention & control*
  • Withania*


  • Antioxidants
  • Bromobenzenes
  • Plant Extracts
  • bromobenzene