Nocardioides soli sp. nov., a bacterium isolated from a mountain soil

Antonie Van Leeuwenhoek. 2014 Aug;106(2):271-8. doi: 10.1007/s10482-014-0191-7. Epub 2014 May 21.

Abstract

Strain SR-1(T), a Gram-positive, strictly-aerobic, short-rod shaped, non-motile bacterium, was isolated from a mountain soil collected in Seoul Women's University in South Korea. Growth occurred between 15 and 37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and with 0-2 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SR-1(T) belongs to the genus Nocardioides and is closely related to Nocardioides simplex KCTC 9106(T) (96.8 %), Nocardioides caeni MN8(T) (96.7 %), Nocardioides aromaticivorans H-1(T) (96.6 %), and Nocardioides kongijuensis A2-4(T) (96.6 %). Chemotaxonomic data revealed that strain SR-1(T) possesses MK-8(H4) as predominant menaquinone, LL-2,6-diaminopimelic acid as the diagnostic diamino acid, phosphatidylglycerol and diphosphatidylglycerol as predominant polar lipids and iso-C16:0, 10-methyl-C18:0, and C18:1 ω9c are major fatty acids. The DNA G+C content of the strain SR-1(T) was 72.4 mol%. Based on polyphasic evidence, strain SR-1(T) (= KEMC 9004-134(T) = JCM 19684(T)) should be classified as the type strain of a novel Nocardioides species, for which the name Nocardioides soli sp. nov. is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification*
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Actinobacteria / physiology
  • Aerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • Cell Wall / chemistry
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Fatty Acids / analysis
  • Humans
  • Hydrogen-Ion Concentration
  • Korea
  • Locomotion
  • Molecular Sequence Data
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Soil Microbiology*
  • Temperature
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Sodium Chloride
  • Diaminopimelic Acid

Associated data

  • GENBANK/KF955611