Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor

FEBS Lett. 2014 Jun 13;588(13):2133-40. doi: 10.1016/j.febslet.2014.04.049. Epub 2014 May 17.


Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially through intracellular protein interactions. Using affinity isolation, we identified 6 of the 8 components of the chaperonin-containing TCP-1 (CCT) complex bound to LOX-1 cytoplasmic domain, which we verified by coimmunoprecipitation and immunostaining in human umbilical vein endothelial cells. We found that the interaction between CCT and LOX-1 is direct and ATP-dependent and that OxLDL suppressed this interaction. Understanding the association between LOX-1 and the CCT complex may facilitate the design of novel therapies for cardiovascular disease.

Keywords: Atherosclerosis; CCT complex; Lectin-like oxidized low-density lipoprotein receptor; Protein–protein interaction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Chaperonin Containing TCP-1 / chemistry*
  • Chaperonin Containing TCP-1 / genetics
  • Chaperonin Containing TCP-1 / metabolism*
  • Gene Knockdown Techniques
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Ligands
  • Lipoproteins, LDL / metabolism
  • Molecular Sequence Data
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / metabolism
  • Protein Interaction Domains and Motifs
  • Protein Subunits
  • RNA, Small Interfering / genetics
  • Scavenger Receptors, Class E / chemistry*
  • Scavenger Receptors, Class E / genetics
  • Scavenger Receptors, Class E / metabolism*


  • Ligands
  • Lipoproteins, LDL
  • Multiprotein Complexes
  • OLR1 protein, human
  • Protein Subunits
  • RNA, Small Interfering
  • Scavenger Receptors, Class E
  • TCP1 protein, human
  • oxidized low density lipoprotein
  • Chaperonin Containing TCP-1