Spatial and temporal expression of RA70/Scap2 in the developing neural tube

Neurosci Lett. 2014 Jul 25;576:1-5. doi: 10.1016/j.neulet.2014.05.013. Epub 2014 May 15.

Abstract

Src kinase-associated phosphoprotein 2 (Ra70/scap2), which was originally isolated as a retinoic acid (RA)-induced gene, associates with molecules that modulate integrin-survival signals. Although RA is essential for vertebrate organogenesis in the posterior region, little is known about the biological role of RA70/Scap2 during development. In the present study, we demonstrate that Ra70/scap2 mRNA is temporally expressed during the RA-induced neuronal differentiation of P19 embryonic carcinoma cells. Homozygous knockout mice in which the Ra70/scap2 gene was replaced with LacZ exhibited embryonic lethality, while heterozygous mice displayed preferential expression of LacZ in posterior neural tissues, including the neural tube and hindbrain during development (E7.5-11.5), but not the forebrain. Ra70/scap2 was expressed in the ependymal layer and ventricular zone in the neural tube, where neuroepithelial cells and neuroblasts with proliferation capacity are localized, respectively. Thus, RA70/Scap2 may be necessary for RA-induced neuronal differentiation from the posterior neuroectoderm.

Keywords: Integrin; Neural tube; Retinoic acid; SKAP55; Scap.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Embryo, Nonmammalian / metabolism
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Mice, Knockout
  • Neural Tube / embryology
  • Neural Tube / metabolism*
  • RNA, Messenger / metabolism

Substances

  • Intracellular Signaling Peptides and Proteins
  • RNA, Messenger
  • src kinase associated phosphoprotein 2