Fetal exposure to maternal inflammation does not affect postnatal development of genetically-driven ileitis and colitis

PLoS One. 2014 May 21;9(5):e98237. doi: 10.1371/journal.pone.0098237. eCollection 2014.

Abstract

Background: Chronic inflammatory disorders have been increasing in incidence over the past decades following geographical patterns of industrialization. Fetal exposure to maternal inflammation may alter organ functions and the offspring's disease risk. We studied the development of genetically-driven ileitis and colitis in response to maternal inflammation using mouse models.

Methods: Disease susceptible (TnfΔARE/+ and IL10-/-) and disease-free (Tnf+/+ and IL10-/+) offspring were raised in inflamed and non-inflamed dams. Ileal, caecal and colonic pathology was evaluated in the offspring at 8 or 12 weeks of age. Ly6G-positive cells in inflamed sections from the distal ileum and distal colon were analysed by immunofluorescence microscopy. Gene expression of pro-inflammatory cytokines was measured in whole tissue specimens by quantitative PCR. Microarray analyses were performed on laser microdissected intestinal epithelium. Caecal bacterial communities were assessed by Illumina sequencing of 16S rRNA amplicons.

Results: Disease severity, the number of infiltrated neutrophils as well as Tnf and Il12p40 mRNA expression were independent of maternal inflammation in the offspring of mouse models for ileitis (TnfΔARE/+) and colitis (IL10-/-). Although TNF-driven maternal inflammation regulated 2,174 (wild type) and 3,345 (TnfΔARE/+) genes in the fetal epithelium, prenatal gene expression patterns were completely overwritten after birth. In addition, co-housing experiments revealed no change in phylogenetic diversity of the offspring's caecal microbiota in response to maternal inflammation. This is independent of the offspring's genotype before and after the onset of tissue pathology.

Conclusions: Disease risk and activity in mouse models of chronic ileitis and colitis was independent of the fetal exposure to maternal inflammation. Likewise, maternal inflammation did not alter the diversity and composition of offspring's caecal microbiota, clearly demonstrating that changes of the gene expression program in the fetal gut epithelium were not relevant for the development of chronic inflammatory disorders in the gut.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colitis / genetics*
  • Colitis / pathology*
  • DNA Primers
  • Disease Models, Animal
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Ileitis / genetics*
  • Ileitis / pathology*
  • Inflammation / pathology*
  • Interleukin-10 / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • Neutrophils / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Phylogeny
  • Pregnancy
  • Prenatal Exposure Delayed Effects*
  • RNA, Ribosomal, 16S / metabolism
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S
  • Tumor Necrosis Factor-alpha
  • Interleukin-10

Associated data

  • GEO/GSE44433

Grant support

This work was supported by the ‘Nutritional Adaptation and Epigenetic Mechanisms’ program of ZIEL - Research Center for Nutrition and Food Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.