Detection of opsonic antibodies against Enterococcus faecalis cell wall carbohydrates in immune globulin preparations

Infection. 2014 Aug;42(4):749-55. doi: 10.1007/s15010-014-0628-y. Epub 2014 May 23.


Three different commercially available polyvalent immune globulins (IG) were investigated for the existence of antibodies against cell wall carbohydrates of four different E. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). All three IG preparations contained antibodies against all four serotypes (CPS-A to CPS-D). However, only one of the three IG preparations showed opsonic killing against all four serotypes. Average killing was higher against serotypes A and B (72 and 79 %, respectively) than against serotypes C and D (30 and 37 %, respectively). Such IG preparations could play a role as an adjuvant therapeutic option in life-threatening infections with E. faecalis, particularly when resistant strains are involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / immunology*
  • Cell Wall / immunology*
  • Enterococcus faecalis / classification
  • Enterococcus faecalis / immunology*
  • Enterococcus faecalis / physiology
  • Gram-Positive Bacterial Infections / therapy
  • Humans
  • Immunoglobulins / immunology*
  • Immunotherapy / methods
  • Microbial Viability
  • Opsonin Proteins / immunology*
  • Pharmaceutical Preparations*
  • Serogroup


  • Carbohydrates
  • Immunoglobulins
  • Opsonin Proteins
  • Pharmaceutical Preparations