Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration

Cell. 2014 May 22;157(5):1146-59. doi: 10.1016/j.cell.2014.03.045.


E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-specific RNAi, photoactivatable Rac, and morphodynamic profiling, to interrogate how E-cadherin contributes to collective migration of cells between other cells. Using the Drosophila ovary as a model, we found that adhesion between border cells and their substrate, the nurse cells, functions in a positive feedback loop with Rac and actin assembly to stabilize forward-directed protrusion and directionally persistent movement. Adhesion between individual border cells communicates direction from the lead cell to the followers. Adhesion between motile cells and polar cells holds the cluster together and polarizes each individual cell. Thus, E-cadherin is an integral component of the guidance mechanisms that orchestrate collective chemotaxis in vivo.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Cadherins / metabolism*
  • Cell Adhesion
  • Cell Movement*
  • Chemotaxis
  • Cytological Techniques
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / metabolism
  • Female
  • Molecular Sequence Data
  • Ovary / cytology
  • rac GTP-Binding Proteins / metabolism


  • Cadherins
  • Drosophila Proteins
  • shg protein, Drosophila
  • rac GTP-Binding Proteins

Associated data

  • GENBANK/KJ826435
  • GENBANK/KJ826436