Fluorogenic probes for live-cell imaging of the cytoskeleton

doi:10.1038/nmeth.2972

. 2014 Jul;11(7):731-3.

doi: 10.1038/nmeth.2972. Epub 2014 May 25.

Fluorogenic probes for live-cell imaging of the cytoskeleton

Gražvydas Lukinavičius¹, Luc Reymond², Elisa D'Este³, Anastasiya Masharina⁴, Fabian Göttfert³, Haisen Ta³, Angelika Güther⁵, Mathias Fournier⁶, Stefano Rizzo⁷, Herbert Waldmann⁷, Claudia Blaukopf⁸, Christoph Sommer⁸, Daniel W Gerlich⁸, Hans-Dieter Arndt⁵, Stefan W Hell³, Kai Johnsson⁹

Affiliations

¹ 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].
² 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].
³ Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
⁴ Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
⁵ Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
⁶ Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
⁷ Max Planck Institute of Molecular Physiology, Dortmund, Germany.
⁸ Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
⁹ 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.

PMID: 24859753
DOI: 10.1038/nmeth.2972

Fluorogenic probes for live-cell imaging of the cytoskeleton

Gražvydas Lukinavičius et al. Nat Methods. 2014 Jul.

. 2014 Jul;11(7):731-3.

doi: 10.1038/nmeth.2972. Epub 2014 May 25.

Authors

Affiliations

¹ 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].
² 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].
³ Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
⁴ Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
⁵ Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
⁶ Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
⁷ Max Planck Institute of Molecular Physiology, Dortmund, Germany.
⁸ Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
⁹ 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.

PMID: 24859753
DOI: 10.1038/nmeth.2972

Abstract

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

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[1] Cell. 2011 Feb 4;144(3):364-75 - PubMed

[2] Cell. 2011 Feb 4;144(3):364-75 - PubMed

[3] Cell. 2009 Feb 6;136(3):473-84 - PubMed

[4] Cell. 2009 Feb 6;136(3):473-84 - PubMed

[5] Biophys J. 2013 Jul 2;105(1):L01-3 - PubMed

[6] Biophys J. 2013 Jul 2;105(1):L01-3 - PubMed

[7] J Microsc. 2000 May;198(Pt 2):82-7 - PubMed

[8] J Microsc. 2000 May;198(Pt 2):82-7 - PubMed

[9] J Biol Chem. 2005 Feb 4;280(5):3928-37 - PubMed

[10] J Biol Chem. 2005 Feb 4;280(5):3928-37 - PubMed

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Fluorogenic probes for live-cell imaging of the cytoskeleton

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Fluorogenic probes for live-cell imaging of the cytoskeleton

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