Hybrid capture II and PapilloCheck® tests for detection of anal high-risk human papillomavirus

Rev Soc Bras Med Trop. Mar-Apr 2014;47(2):227-30. doi: 10.1590/0037-8682-0081-2012.


Introduction: This study evaluated the level of concordance between hybrid capture II (HCII) and PapilloCheck® for the detection of high-risk human papillomavirus (HPV) in anal samples.

Methods: Anal cell samples collected from 42 human immunodeficiency virus (HIV)+ patients were analyzed.

Results: Considering only the 13 high-risk HPV types that are detectable by both tests, HCII was positive for 52.3% of the samples, and PapilloCheck® was positive for 52.3%. The level of concordance was 80.9% (Kappa = 0.61).

Conclusions: Good concordance was observed between the tests for the detection of high-risk HPV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anal Canal / virology*
  • Anus Diseases / diagnosis*
  • Anus Diseases / virology
  • DNA, Viral / genetics*
  • Female
  • Genotype
  • HIV Infections*
  • Humans
  • Nucleic Acid Amplification Techniques / methods
  • Papillomaviridae / genetics*
  • Papillomavirus Infections / diagnosis*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity


  • DNA, Viral
  • Reagent Kits, Diagnostic