Ultrasound assessment of the diaphragm: Preliminary study of a canine model of X-linked myotubular myopathy

Muscle Nerve. 2014 Oct;50(4):607-9. doi: 10.1002/mus.24294. Epub 2014 Aug 30.


Introduction: We tested the feasibility of using neuromuscular ultrasound for non-invasive real-time assessment of diaphragmatic structure and function in a canine model of X-linked myotubular myopathy (XLMTM).

Methods: Ultrasound images in 3 dogs [wild-type (WT), n=1; XLMTM untreated, n=1; XLMTM post-AAV8-mediated MTM1 gene replacement, n=1] were analyzed for diaphragm thickness, change in thickness with respiration, muscle echogenicity, and diaphragm excursion amplitude during spontaneous breathing.

Results: Quantitative parameters of diaphragm structure were different among the animals. WT diaphragm was thicker and less echogenic than the XLMTM control, whereas the diaphragm measurements of the MTM1-treated XLMTM dog were comparable to those of the WT dog.

Conclusions: This pilot study demonstrates the feasibility of using ultrasound for quantitative assessment of the diaphragm in a canine model. In the future, ultrasonography may replace invasive measures of diaphragm function in canine models and in humans for non-invasive respiratory monitoring and evaluation of neuromuscular disease.

Keywords: canine; diaphragm; respiratory failure; ultrasonography; ultrasound.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diaphragm / diagnostic imaging*
  • Diaphragm / pathology
  • Diaphragm / physiopathology*
  • Disease Models, Animal
  • Dogs
  • Male
  • Myopathies, Structural, Congenital / pathology*
  • Myopathies, Structural, Congenital / veterinary
  • Pilot Projects
  • Ultrasonography