Measurement of fatty acid oxidation rates in animal tissues and cell lines

Methods Enzymol. 2014;542:391-405. doi: 10.1016/B978-0-12-416618-9.00020-0.

Abstract

While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.

Keywords: Acid-soluble metabolite; Cell lysate; Radiolabeling; Xenografts; β-Oxidation CO(2).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biochemistry / instrumentation
  • Biochemistry / methods*
  • Carbon Radioisotopes
  • Cell Line
  • Fatty Acids / analysis*
  • Fatty Acids / metabolism*
  • Oxidation-Reduction
  • Palmitates
  • Tumor Cells, Cultured

Substances

  • Carbon Radioisotopes
  • Fatty Acids
  • Palmitates