Evaluation of cardiovascular toxicity of carbon nanotubes functionalized with sodium hyaluronate in oral regenerative medicine

Braz J Med Biol Res. 2014 Jul;47(7):560-6. doi: 10.1590/1414-431x20143894. Epub 2014 May 23.


It has been demonstrated that carbon nanotubes (CNTs) associated with sodium hyaluronate (HY-CNTs) accelerate bone repair in the tooth sockets of rats. Before clinical application of HY-CNTs, it is important to assess their biocompatibility. Moreover, cardiac toxicity may be caused by the translocation of these particles to the blood stream. The aim of this study was to evaluate possible changes in cardiovascular function in male Wistar rats whose tooth sockets were treated with either CNTs or HY-CNTs (100 μg/mL, 0.1 mL). Blood pressure and heart rate were monitored in conscious rats 7 days after treatment. Cardiac function was evaluated using the Langendorff perfusion technique. The data showed no changes in blood pressure or heart rate in rats treated with either CNTs or HY-CNTs, and no significant changes in cardiac function were found in any of the groups. To confirm these findings, experiments were conducted in rats injected intraperitoneally with a high concentration of either CNTs or HY-CNTs (0.75 mg/kg). The same parameters were analyzed and similar results were observed. The results obtained 7 days following injection indicate that the administration of low concentrations of CNTs or HY-CNTs directly into tooth sockets did not cause any significant change in cardiovascular function in the rats. The present findings support the possibility of using these biocomposites in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure / drug effects*
  • Cardiovascular Physiological Phenomena
  • Coronary Circulation / drug effects
  • Heart Function Tests / drug effects
  • Heart Rate / drug effects*
  • Hyaluronic Acid / administration & dosage
  • Hyaluronic Acid / toxicity*
  • Injections, Intraperitoneal
  • Male
  • Materials Testing
  • Microscopy, Electron, Transmission
  • Mouth / surgery
  • Nanotubes, Carbon / toxicity*
  • Perfusion / methods
  • Rats, Wistar
  • Spectrum Analysis
  • Thermogravimetry
  • Tooth Extraction
  • Tooth Socket / drug effects*
  • Tooth Socket / surgery


  • Nanotubes, Carbon
  • Hyaluronic Acid