Dibenzocyclooctadiene lignans from Schisandra chinensis and their inhibitory activity on NO production in lipopolysaccharide-activated microglia cells

Phytochemistry. 2014 Aug:104:72-8. doi: 10.1016/j.phytochem.2014.04.014. Epub 2014 May 23.

Abstract

Four dibenzocyclooctadiene lignans, schisanchinins A-D, and 10 known compounds were isolated from the EtOAc extract of fruits of Schisandra chinensis (Turcz.) Baill. Structures of compounds 1-4 were elucidated using a combination of spectroscopic techniques, including MS, UV and IR, NMR ((1)H NMR, (13)C NMR, HMQC, HMBC). The stereochemistry of the chiral centers and the biphenyl configuration were determined using NOESY, as well as analysis of CD spectra. In vitro activity assays showed that 11 of the 14 compounds exhibited inhibitory activity on lipopolysaccharide (LPS)-induced NO release in primary murine BV2 microglia cells.

Keywords: Dibenzocyclooctadiene lignans; Inhibitory activity on lipopolysaccharide (LPS)-induced NO release; Schisandra chinensis; Schisandraceae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cyclooctanes / chemistry
  • Cyclooctanes / isolation & purification
  • Cyclooctanes / pharmacology*
  • Fruit / chemistry
  • Lignans / chemistry
  • Lignans / isolation & purification
  • Lignans / pharmacology*
  • Lipopolysaccharides / metabolism
  • Mice
  • Microglia / drug effects
  • Molecular Structure
  • Nitric Oxide / metabolism*
  • Nitrites / analysis
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Schisandra / chemistry*

Substances

  • Cyclooctanes
  • Lignans
  • Lipopolysaccharides
  • Nitrites
  • Plant Extracts
  • dibenzocyclooctadiene lignan
  • Nitric Oxide