Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats

J Pak Med Assoc. 2014 Mar;64(3):260-5.


Objective: To investigate the potential protective effects of Rosmarinic acid (RA) on rats exposed to ischaemia/reperfusion renal injury.

Methods: The prospective study was conducted at Abant Izzet Baysal University, Turkey, and comprised 21 male Spraque Dawley rats weighing 250-270g each. They were divided into three equal groups. Unilaterally nephrectomised rats were subjected to 60 minutes of left renal ischaemia followed by 60 minutes of reperfusion. Group 1 had sham-operated animals; group 2 had ischaemia/reperfusion untreated animals; and group 3 had ischaemia/reperfusion animals treated with rosmarinic acid. Serum creatinine, blood urea nitrogen, tissue malondialdehyde, glutathione peroxidase, superoxide dismutase and myeloperoxidase (MPO) activities, and light microscopic findings were evaluated. SPSS 17 was used for statistical analysis.

Results: Treatment of rats with rosmarinic acid produced a reduction in the serum levels of creatinine and blood urea nitrogen compared to the other groups. However, no statistically significant difference was found. The levels of malondialdehyde and myeloperoxidase were decreased in the renal tissue of group 3, while glutathione peroxidose and superoxide dismutase levels remained unchanged. The injury score decreased in the treatment group rats compared to the untreated group. Rosmarinic acid significantly decreased focal glomerular necrosis, dilatation of Bowman's capsule, degeneration of tubular epithelium, necrosis in tubular epithelium, and tubular dilatation.

Conclusions: Rosmarinic acid prevented ischaemia/reperfusion injury in the kidneys by decreasing oxidative stress.

MeSH terms

  • Animals
  • Blood Urea Nitrogen
  • Cinnamates / pharmacology*
  • Creatinine / blood
  • Depsides / pharmacology*
  • Kidney Diseases / blood*
  • Kidney Diseases / prevention & control*
  • Male
  • Malondialdehyde / blood
  • Nephrectomy
  • Oxidative Stress
  • Peroxidase / blood
  • Prospective Studies
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / blood*
  • Reperfusion Injury / prevention & control*
  • Rosmarinic Acid


  • Cinnamates
  • Depsides
  • Malondialdehyde
  • Creatinine
  • Peroxidase