Bone-marrow mesenchymal stem cell transplantation to treat diabetic nephropathy in tree shrews

Cell Biochem Funct. 2014 Jul;32(5):453-63. doi: 10.1002/cbf.3037. Epub 2014 May 28.


Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin. 4',6-Diamidino-2-phenylindole labelled BM-MSCs were injected into tree shrews. The DN tree shrew model was successfully established. Blood glucose was significantly increased ( p < 0.01) during the entire experiment. DN tree shrews showed dyslipidemia, insulin resistance and increased 24-h proteinuria. At 21 days after BM-MSC transplantation, glucose and levels of triglycerides, total cholesterol and 24-h urine volume were lower than in tree shrews with DN alone ( p < 0.01) but were still higher than control values ( p < 0.01). Levels of creatinine and urea nitrogen as well as 24-h proteinuria were lower for DN tree shrews with BM-MSCs transplantation than DN alone ( p < 0.05). High-sugar and high-fat diet combined with STZ injection can induce a tree shrew model of DN. BM-MSCs injection can home to damaged kidneys and pancreas, for reduced 24-h proteinuria and improved insulin resistance.

Keywords: DAPI; bone-marrow mesenchymal stem cells; diabetic nephropathy; transplantation; tree shrews.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Blood Urea Nitrogen
  • Bone Marrow Cells / cytology*
  • Cholesterol / blood
  • Creatinine / blood
  • Diabetic Nephropathies / chemically induced
  • Diabetic Nephropathies / pathology
  • Diabetic Nephropathies / therapy*
  • Diet, High-Fat
  • Disease Models, Animal
  • Glomerular Filtration Rate
  • Glycation End Products, Advanced / blood
  • Insulin / blood
  • Kidney / pathology
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology*
  • Pancreas / pathology
  • Streptozocin / toxicity
  • Triglycerides / blood
  • Tupaiidae


  • Blood Glucose
  • Glycation End Products, Advanced
  • Insulin
  • Triglycerides
  • Streptozocin
  • Cholesterol
  • Creatinine