Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice

Int Immunopharmacol. 2014 Aug;21(2):406-11. doi: 10.1016/j.intimp.2014.05.018. Epub 2014 May 24.


Hesperidin (HDN) is a citrus bioflavonoid, which widely exists in many plants. Previous researches have proved that HDN has several functions such as anti-oxidant, anti-tumor, anti-inflammatory, immune regulation and so on. In the present study, we explored the protective effects of HDN on concanavalin A (Con A)-induced hepatic injury. Acute hepatic injury model was established successfully by intravenous administration of Con A (15 mg/kg) in male C57BL/6 mice, and HDN was pretreated for 10 days before Con A challenge. It was found that the hepatic injury was notably improved in HDN pretreated mice. Furthermore, hepatic oxidative stress and the production of proinflammatory cytokines including TNF-α and IFN-γ were decreased by HDN pretreatment. More importantly, compared with Con A-treated mice, the expression and releasing of HMGB1 and T-cell activation were markedly reduced in HDN pretreated mice. Thus, these results suggest that HDN protects mice from Con A-induced hepatic injury by suppressing hepatocyte oxidative stress, producing cytokines, expressing and releasing HMGB1 and activating T cells.

Keywords: Concanavalin A; HMGB1; Hepatic injury; Hesperidin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Chemical and Drug Induced Liver Injury / metabolism
  • Concanavalin A / adverse effects*
  • HMGB1 Protein / metabolism
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Hesperidin / pharmacology*
  • Interferon-gamma / metabolism
  • Liver / drug effects
  • Liver / metabolism
  • Lymphocyte Activation / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oxidative Stress / drug effects
  • Protective Agents / pharmacology*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism
  • Tumor Necrosis Factor-alpha / metabolism


  • Anti-Inflammatory Agents
  • HMGB1 Protein
  • HMGB1 protein, mouse
  • Protective Agents
  • Tumor Necrosis Factor-alpha
  • Concanavalin A
  • Interferon-gamma
  • Hesperidin