The active site of cytochrome P-450 nifedipine oxidase: a model-building study

J Mol Graph. 1989 Dec;7(4):206-11. doi: 10.1016/0263-7855(89)80004-9.

Abstract

A model of the active site of cytochrome P-450 nifedipine oxidase is built on the basis of sequence homology with cytochrome P-450CAM. Substrates are docked into the binding pocket, and molecular mechanical energy minimization is performed to analyze the forces between the substrates and the enzyme.

MeSH terms

  • Binding Sites
  • Camphor 5-Monooxygenase
  • Computer Graphics
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System / chemistry*
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Mixed Function Oxygenases / chemistry*
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism
  • Models, Chemical*
  • Mutation
  • Quinidine / metabolism
  • Sequence Homology, Nucleic Acid
  • Testosterone / metabolism

Substances

  • Testosterone
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • CYP3A protein, human
  • Cytochrome P-450 CYP3A
  • Camphor 5-Monooxygenase
  • Quinidine