Overexpression of the putative extracytoplasmic function sigma (σ) factor FujE enhances FK506 production in Streptomyces sp. strain KCCM 11116P

Can J Microbiol. 2014 Jun;60(6):363-9. doi: 10.1139/cjm-2014-0166. Epub 2014 Apr 22.

Abstract

The role of the putative extracytoplasmic function sigma (σ) factor FujE, which has not been characterized as a member of the FK506 biosynthetic gene cluster, on FK506 production was identified by gene deletion, overexpression, and transcription analysis experiments in Streptomyces sp. strain KCCM 11116P. Inactivation of fujE had no effect on FK506 production, growth, or morphological differentiation. Overexpression of fujE with integrative vectors increased FK506 production by 2.87-fold (24.5 ± 1.4 mg·L(-1)) compared with the wild type (8.5 ± 0.5 mg·L(-1)). Semiquantitative reverse transcription-polymerase chain reaction analysis indicated that the overexpression of fujE stimulates the transcription of the FK506 biosynthetic genes. These results demonstrated that fujE is a new member of the FK506 biosynthetic gene cluster.

Keywords: ECF sigma factor; FK506; FK506 biosynthetic gene; facteur sigma FEC; gene overexpression; gène de la biosynthèse du FK506; surexpression génique.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Deletion
  • Gene Expression Regulation, Bacterial*
  • Immunosuppressive Agents / metabolism*
  • Multigene Family*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sigma Factor / genetics
  • Sigma Factor / metabolism*
  • Streptomyces / genetics
  • Streptomyces / metabolism*
  • Tacrolimus / metabolism*
  • Transcription, Genetic

Substances

  • Immunosuppressive Agents
  • Sigma Factor
  • Tacrolimus