Pre and post lipocryolysis thermic conditioning enhances rat adipocyte destruction

Cryo Letters. Mar-Apr 2014;35(2):154-60.

Abstract

Background: New knowledge about crystallization vs. lipid-to-gel transition has surfaced recently, since some of the latest publications on lipocryolysis have focused on its action mechanism. As a result, new opportunities for technical improvements and clinical outcome optimization have opened up. The food industry has been working with lipid crystal polymorphisms for decades, and tempering seems to be the easiest method of external conditioning, in addition to being harmless.

Objective: Evaluate if pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction.

Methods: Several temperature treatment patterns (TTP) were applied to isolated rat adipocytes. The survival of the adipocytes exposed to the different TTPs and the formation of crystals in the surviving adipocytes were assessed and analyzed.

Result: Pre and post lipocryolysis thermic conditioning changed lipocryolyisis crystallization process and showed an enhancement in adipocyte destruction that could represent an important step in improving clinical results.

Conclusions: pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / chemistry
  • Adipocytes / cytology*
  • Adipose Tissue, White / chemistry
  • Adipose Tissue, White / cytology*
  • Animals
  • Cell Death
  • Cell Survival
  • Cold Temperature
  • Crystallization
  • Hot Temperature
  • Male
  • Membrane Lipids / chemistry*
  • Phase Transition
  • Primary Cell Culture
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Membrane Lipids