Antiviral activity of aloe-emodin against influenza A virus via galectin-3 up-regulation

Eur J Pharmacol. 2014 Sep 5;738:125-32. doi: 10.1016/j.ejphar.2014.05.028. Epub 2014 May 27.

Abstract

Novel influenza A H7N9 virus, which emerged in 2013, and highly pathogenic H5N1 virus, identified since 2003, pose challenges to public health and necessitate quest for new anti-influenza compounds. Anthraquinone derivatives like aloe-emodin, emodin and chrysophanol, reportedly exhibit antiviral activity. This study probes their inhibitory mechanism and effect against influenza A virus. Of three anthraquinone derivatives, aloe-emodin, with a lower cytotoxicity showed concentration-dependently reducing virus-induced cytopathic effect and inhibiting replication of influenza A in MDCK cells. 50% inhibitory concentration value of aloe-emodin on virus yield was less than 0.05 μg/ml. Proteomics and Western blot of MDCK cells indicated aloe-emodin up-regulating galectin-3, and thioredoxin as well as down-regulating nucleoside diphosphate kinase A. Western blot and quantitative PCR confirmed aloe-emodin up-regulating galectin-3 expression; recombinant galectin-3 augmented expression of antiviral genes IFN-β, IFN-γ, PKR and 2'5',-OAS in infected cells, agreeing with expression pattern of those treated with aloe-emodin. Galectin-3 also inhibited influenza A virus replication. Proteomic analysis of treated cells indicated galectin-3 up-regulation as one anti-influenza A virus action by aloe-emodin. Since galectin-3 exhibited cytokine-like regulatory actions via JAK/STAT pathways, aloe-emodin also restored NS1-inhibited STAT1-mediated antiviral responses in transfected cells: e.g., STAT1 phosphorylation of interferon (IFN) stimulation response element (ISRE)-driven promoter, RNA-dependent protein kinase (PKR) and 2'5',-oligoadenylate synthetase (2'5',-OAS) expression. Treatment with aloe-emodin could control influenza infection in humans.

Keywords: Aloe-emodin; Anthraquinone; Galectin-3; Influenza A virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthraquinones / pharmacology*
  • Antiviral Agents / pharmacology*
  • Dogs
  • Galectin 3 / genetics*
  • Humans
  • Influenza A virus / drug effects*
  • Influenza A virus / physiology
  • Interferon Type I / pharmacology
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Madin Darby Canine Kidney Cells
  • Proteomics
  • STAT Transcription Factors / metabolism
  • Signal Transduction / drug effects
  • Up-Regulation / drug effects*
  • Viral Nonstructural Proteins / antagonists & inhibitors
  • Viral Nonstructural Proteins / genetics
  • Virus Replication / drug effects

Substances

  • Anthraquinones
  • Antiviral Agents
  • Galectin 3
  • INS1 protein, influenza virus
  • Interferon Type I
  • STAT Transcription Factors
  • Viral Nonstructural Proteins
  • aloe emodin
  • JNK Mitogen-Activated Protein Kinases