Influenza entry pathways in polarized MDCK cells

Biochem Biophys Res Commun. 2014 Jul 18;450(1):234-9. doi: 10.1016/j.bbrc.2014.05.095. Epub 2014 May 27.


In non-polarized cell culture models, influenza virus has been shown to enter host cells via multiple endocytic pathways, including classical clathrin-mediated endocytic routes (CME), clathrin- and caveolae-independent routes and macropinocytosis. However, little is known about the entry route of influenza virus in differentiated epithelia, in vivo site of infection for influenza virus. Here, we show that in polarized Madin-Darby canine kidney type II (MDCK II) cells, influenza virus has a specific utilization of the clathrin-mediated endocytic pathway and requires Eps15 for host cell entry.

Keywords: Clathrin; Influenza virus; Polarized cell; Virus entry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism*
  • Adaptor Proteins, Vesicular Transport
  • Animals
  • Cell Polarity / physiology*
  • Clathrin / metabolism*
  • Dogs
  • Endocytosis*
  • Influenza A Virus, H1N1 Subtype / physiology*
  • Madin Darby Canine Kidney Cells
  • Signal Transduction*
  • Virus Internalization*


  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport
  • Clathrin
  • EPN2 protein, human