Structure of cellular ESCRT-III spirals and their relationship to HIV budding

Elife. 2014 May 30;3:e02184. doi: 10.7554/eLife.02184.

Abstract

The ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission for trafficking into multivesicular bodies in the endocytic pathway and for the topologically related processes of viral budding and cytokinesis, but how they accomplish this remains unclear. Using deep-etch electron microscopy, we find that endogenous ESCRT-III filaments stabilized by depleting cells of Vps4 create uniform membrane-deforming conical spirals which are assemblies of specific ESCRT-III heteropolymers. To explore functional roles for ESCRT-III filaments, we examine HIV-1 Gag-mediated budding of virus-like particles and find that depleting Vps4 traps ESCRT-III filaments around nascent Gag assemblies. Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral.

Keywords: ESCRT; HIV-1 budding; cell biology; deep-etch EM; endosome; human; multivesicular body; viruses.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biological Transport
  • COS Cells
  • Cell Membrane / metabolism
  • Chlorocebus aethiops
  • Cytokinesis
  • Cytoplasm / metabolism
  • Endosomal Sorting Complexes Required for Transport / chemistry*
  • Gene Products, gag / chemistry
  • HEK293 Cells
  • HIV-1 / chemistry
  • HIV-1 / physiology*
  • HeLa Cells
  • Humans
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Polymers / chemistry
  • Protein Conformation
  • Virus Release*

Substances

  • Endosomal Sorting Complexes Required for Transport
  • Gene Products, gag
  • Polymers