A novel bacterial strain, designated PFS-5(T), was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5(T) was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5(T) in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5a(T) (96.9%), B. humi LMG 22167(T) (96.7%), B. alkalitelluris BA288(T) (96.1%), B. litoralis SW-211(T) (96.0%), and B. luteolus YIM93174(T) (95.5%). The major polar lipids of PFS-5(T) were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5(T) represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strai PFS-5(T) = KACC 17469(T) = JCM19333(T)).