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. 2014 Aug;32(8):819-21.
doi: 10.1038/nbt.2925. Epub 2014 Jun 1.

Genome Editing in the Human Malaria Parasite Plasmodium Falciparum Using the CRISPR-Cas9 System

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Genome Editing in the Human Malaria Parasite Plasmodium Falciparum Using the CRISPR-Cas9 System

Mehdi Ghorbal et al. Nat Biotechnol. .

Abstract

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.

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