Synaptotagmin 11 interacts with components of the RNA-induced silencing complex RISC in clonal pancreatic β-cells

FEBS Lett. 2014 Jun 27;588(14):2217-22. doi: 10.1016/j.febslet.2014.05.031. Epub 2014 May 29.


Synaptotagmins are two C2 domain-containing transmembrane proteins. The function of calcium-sensitive members in the regulation of post-Golgi traffic has been well established whereas little is known about the calcium-insensitive isoforms constituting half of the protein family. Novel binding partners of synaptotagmin 11 were identified in β-cells. A number of them had been assigned previously to ER/Golgi derived-vesicles or linked to RNA synthesis, translation and processing. Whereas the C2A domain interacted with the Q-SNARE Vti1a, the C2B domain of syt11 interacted with the SND1, Ago2 and FMRP, components of the RNA-induced silencing complex (RISC). Binding to SND was direct via its N-terminal tandem repeats. Our data indicate that syt11 may provide a link between gene regulation by microRNAs and membrane traffic.

Keywords: ER-Golgi; Pancreatic Islets; RISC; SNARE; Secretory pathway; Synaptotagmins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Argonaute Proteins / metabolism
  • Cell Line
  • Endonucleases
  • Fragile X Mental Retardation Protein / metabolism
  • Insulin-Secreting Cells / metabolism*
  • Male
  • Mice
  • MicroRNAs / physiology
  • Nuclear Proteins / metabolism
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Transport
  • RNA Interference
  • RNA-Induced Silencing Complex / metabolism*
  • Rats
  • Rats, Wistar
  • Synaptotagmins / metabolism*


  • Ago2 protein, mouse
  • Argonaute Proteins
  • Fmr1 protein, mouse
  • MicroRNAs
  • Nuclear Proteins
  • RNA-Induced Silencing Complex
  • Syt11 protein, mouse
  • Synaptotagmins
  • Fragile X Mental Retardation Protein
  • Endonucleases
  • Snd1 protein, mouse