Closed-loop optical neural stimulation based on a 32-channel low-noise recording system with online spike sorting

J Neural Eng. 2014 Aug;11(4):046005. doi: 10.1088/1741-2560/11/4/046005. Epub 2014 Jun 3.


Objective: Closed-loop operation of neuro-electronic systems is desirable for both scientific and clinical (neuroprosthesis) applications. Integrating optical stimulation with recording capability further enhances the selectivity of neural stimulation. We have developed a system enabling the local delivery of optical stimuli and the simultaneous electrical measuring of the neural activities in a closed-loop approach.

Approach: The signal analysis is performed online through the implementation of a template matching algorithm. The system performance is demonstrated with the recorded data and in awake rats.

Main results: Specifically, the neural activities are simultaneously recorded, detected, classified online (through spike sorting) from 32 channels, and used to trigger a light emitting diode light source using generated TTL signals.

Significance: A total processing time of 8 ms is achieved, suitable for optogenetic studies of brain mechanisms online.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Amplifiers, Electronic
  • Animals
  • Artifacts
  • Electric Stimulation / instrumentation
  • Electric Stimulation / methods*
  • Male
  • Neural Prostheses*
  • Online Systems
  • Rats
  • Signal Processing, Computer-Assisted
  • Signal-To-Noise Ratio
  • Software