Paraphyly of the Subgenus Sintonius (Diptera, Psychodidae, Sergentomyia): Status of the Malagasy Species. Creation of a New Subgenus and Description of a New Species

PLoS One. 2014 Jun 3;9(6):e98065. doi: 10.1371/journal.pone.0098065. eCollection 2014.

Abstract

During an inventory of Phlebotomine sand flies carried out in Madagascar, we have identified some specimens showing morphological characters related to the subgenus Sintonius of the genus Sergentomyia. We started a molecular study based on cytochrome b mtDNA and on D1-D2 and D8 domains of the rDNA. The sampling includes all the Sergentomyia species available and also S. (Sergentomyia) schwetzi, S. (Parrotomyia) magna, and the following species belonging to the subgenus Sintonius: S. clydei, S. christophersi, S. affinis vorax, S. adleri and S. meilloni. The Sintonius subgenus (sensu Theodor) is paraphyletic. The Malagasy specimens morphologically Sintonius-like are never clustered with the continental Sintonius. We propose a new subgenus to include them: Trouilletomyia subg. nov. Due to the lack of mesanepisternal setae, the species huberti is removed from the genus Phlebotomus and we propose here a new combination: Sergentomyia huberti comb. nov. The male of S. huberti is pinpointed and described for the first time. Lastly, a new species for Science is described on one female: Sergentomyia (Trouilletomyia) boironis n. sp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cytochromes b / genetics
  • DNA, Mitochondrial / genetics
  • DNA, Ribosomal / genetics
  • Female
  • Geography
  • Likelihood Functions
  • Madagascar
  • Male
  • Psychodidae / anatomy & histology*
  • Psychodidae / classification*
  • Species Specificity
  • Terminology as Topic*

Substances

  • DNA, Mitochondrial
  • DNA, Ribosomal
  • Cytochromes b

Grant support

The fieldwork received funding from the Institute of Research for Development, Institut Pasteur de Madagascar, and Volkswagen Stiftung. Laboratory work received funding from: (i) the Program of “Institut Français de la biodiversité/CNRS/AIRD Biodiversité dans les îles de l’Océan Indien'” itself included in the Regional Project “Insectes vecteurs (phlébotomes et moustiques) dans les îles de l’Océan Indien: Madagascar, Seychelles et Comores”, the GDRI “Biodiversité et Développement Durable à Madagascar”; (ii) “Université de Reims Champagne – Ardenne”; and (iii) the Robert S. McNamara Fellowship Program of the World Bank. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.