Selenoprotein S is involved in maintenance and transport of multiprotein complexes

Biochem J. 2014 Sep 15;462(3):555-65. doi: 10.1042/BJ20140076.


SelS (Selenoprotein S) is a selenocysteine-containing protein with roles in ER (endoplasmic reticulum) function and inflammation. It has been implicated in ERAD (ER-associated protein degradation), and clinical studies revealed an association of its promoter polymorphism with cytokine levels and human diseases. However, the pathways and interacting proteins that could shed light on pathogenesis of SelS-associated diseases have not been studied systematically. We performed a large-scale affinity isolation of human SelS and its mutant forms and analysed the proteins that interact with them. All previously known SelS targets and nearly two hundred additional proteins were identified that were remarkably enriched for various multiprotein complexes. Subsequent chemical cross-linking experiments identified the specific interacting sites in SelS and its several targets. Most of these interactions involved coiled-coil domains. The data suggest that SelS participates in intracellular membrane transport and maintenance of protein complexes by anchoring them to the ER membrane.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Cytochrome-B(5) Reductase / metabolism
  • Endoplasmic Reticulum / metabolism*
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Membrane Proteins / metabolism*
  • Molecular Docking Simulation
  • Multiprotein Complexes / metabolism*
  • Nuclear Proteins / metabolism
  • Selenoproteins / metabolism*


  • Membrane Proteins
  • Multiprotein Complexes
  • Nuclear Proteins
  • SELENOS protein, human
  • Selenoproteins
  • CYB5R3 protein, human
  • Cytochrome-B(5) Reductase
  • Adenosine Triphosphatases
  • p97 ATPase