TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes

Open Biol. 2014 Jun;4(6):130108. doi: 10.1098/rsob.130108.

Abstract

Chromosomal instability can arise from defects in chromosome-microtubule attachment. Using a variety of drug treatments, we show that TAO1 kinase is required for ensuring the normal congression of chromosomes. Depletion of TAO1 reduces the density of growing interphase and mitotic microtubules in human cells, showing TAO1's role in controlling microtubule dynamics. We demonstrate the aneugenic nature of chromosome-microtubule attachment defects in TAO1-depleted cells using an error-correction assay. Our model further strengthens the emerging paradigm that microtubule regulatory pathways are important for resolving erroneous kinetochore-microtubule attachments and maintaining the integrity of the genome, regardless of the spindle checkpoint status.

Keywords: kinetochore; microtubule; mitosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomal Instability*
  • Chromosomes / genetics
  • Chromosomes / metabolism*
  • HeLa Cells
  • Humans
  • MAP Kinase Kinase Kinases / metabolism*
  • Protein-Serine-Threonine Kinases
  • Tumor Cells, Cultured

Substances

  • Protein-Serine-Threonine Kinases
  • TAO1 protein kinase
  • MAP Kinase Kinase Kinases