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, 10 (6), e1004401
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Ancient DNA Analysis of 8000 B.C. Near Eastern Farmers Supports an Early Neolithic Pioneer Maritime Colonization of Mainland Europe Through Cyprus and the Aegean Islands

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Ancient DNA Analysis of 8000 B.C. Near Eastern Farmers Supports an Early Neolithic Pioneer Maritime Colonization of Mainland Europe Through Cyprus and the Aegean Islands

Eva Fernández et al. PLoS Genet.

Abstract

The genetic impact associated to the Neolithic spread in Europe has been widely debated over the last 20 years. Within this context, ancient DNA studies have provided a more reliable picture by directly analyzing the protagonist populations at different regions in Europe. However, the lack of available data from the original Near Eastern farmers has limited the achieved conclusions, preventing the formulation of continental models of Neolithic expansion. Here we address this issue by presenting mitochondrial DNA data of the original Near-Eastern Neolithic communities with the aim of providing the adequate background for the interpretation of Neolithic genetic data from European samples. Sixty-three skeletons from the Pre Pottery Neolithic B (PPNB) sites of Tell Halula, Tell Ramad and Dja'de El Mughara dating between 8,700-6,600 cal. B.C. were analyzed, and 15 validated mitochondrial DNA profiles were recovered. In order to estimate the demographic contribution of the first farmers to both Central European and Western Mediterranean Neolithic cultures, haplotype and haplogroup diversities in the PPNB sample were compared using phylogeographic and population genetic analyses to available ancient DNA data from human remains belonging to the Linearbandkeramik-Alföldi Vonaldiszes Kerámia and Cardial/Epicardial cultures. We also searched for possible signatures of the original Neolithic expansion over the modern Near Eastern and South European genetic pools, and tried to infer possible routes of expansion by comparing the obtained results to a database of 60 modern populations from both regions. Comparisons performed among the 3 ancient datasets allowed us to identify K and N-derived mitochondrial DNA haplogroups as potential markers of the Neolithic expansion, whose genetic signature would have reached both the Iberian coasts and the Central European plain. Moreover, the observed genetic affinities between the PPNB samples and the modern populations of Cyprus and Crete seem to suggest that the Neolithic was first introduced into Europe through pioneer seafaring colonization.

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Map of the spread of Neolithic farming cultures in Europe.
Shadings represent isochronous Neolithic archaeological cultures and black lines frontier zones between them. Analyzed sites in the Fertile Crescent are also located in the map. All dates are in years B.C.
Figure 2
Figure 2. Contour map displaying the percentage of individuals of the database carrying PPNB haplotypes.
Only populations with clear geographic distribution were included. Gradients indicate the degree of similarity between PPNB and modern populations (dark: high; clear: small).
Figure 3
Figure 3. Plot of the two first principal components of the PCA-HCA performed using population haplogroup frequencies.
(A) General plot. (B) Zoom plot of Clusters 1, 2 and 3. Population grouping in 6 clusters after HCA is indicated by colors: Cluster 1 (green), Cluster 2 (red), Cluster 3 (orange), Cluster 4 (light blue), Cluster 5 (grey), Cluster 6 (dark blue). Population labels are described in Table S4.
Figure 4
Figure 4. Contour map of Fst distances between the PPNB population and modern populations of the database.
Only populations with clear geographic distribution were included. Gradients indicate genetic distance between the PPNB and the modern populations (dark: small; clear: high).

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References

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Grant support

The work presented here was funded with the following research projects: BMC2002-2741, CGL2006-07828 and CGL2009-07959 (Ministerio de Ciencia e Innovación, Spanish Government), CCG08-UCM/BIO-3938 (Comunidad Autónoma de Madrid), INCO-MED-ICA3-CT-2002-10022 (European Commission) and I&D-PTDC/HAH/64548/2006 (Fundação para a Ciência e a Tecnologia, Portugal). This work was supported by a Post-Doctoral fellowship from Fundação para a Ciência e a Tecnologia (Portugal) (Ref. SFRH/BPD/69426/2010) and a research contract Juan de la Cierva from the Spanish Government and the European Social Fund (Ref. JCI-2007-56-261) to EF and with a Pre-Doctoral FPU grant (Ref. AP2006-01586) from the Spanish Government to CG. Excavations at the archaeological site of Tell Halula were partially funded with the project HUM2010-18612 (Ministerio de Ciencia e Innovación, Spanish Government). Liverpool John Moores University, Universitat the Barcelona, Universidad Complutense de Madrid and Universitat Autonoma de Barcelona have jointly funded the open access publication costs of this article. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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