Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries

Mil Med. 2014 Jun;179(6):686-94. doi: 10.7205/MILMED-D-13-00360.


This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Blast Injuries / therapy*
  • Cadaver
  • Chondroitin Sulfates / therapeutic use*
  • Collagen / therapeutic use*
  • Corneal Perforation / therapy*
  • Disk Diffusion Antimicrobial Tests
  • Eye Injuries, Penetrating / therapy*
  • Hydrogels
  • Male
  • Membranes, Artificial
  • Polyethylene Glycols / therapeutic use*
  • Rabbits
  • Staphylococcus aureus / drug effects
  • Swine
  • Tissue Adhesives / therapeutic use
  • Vancomycin / administration & dosage


  • Anti-Bacterial Agents
  • Hydrogels
  • Membranes, Artificial
  • Tissue Adhesives
  • Polyethylene Glycols
  • Vancomycin
  • Chondroitin Sulfates
  • Collagen