Cyanidin 3-O-glucoside reduces Helicobacter pylori VacA-induced cell death of gastric KATO III cells through inhibition of the SecA pathway

Int J Med Sci. 2014 May 14;11(7):742-7. doi: 10.7150/ijms.7167. eCollection 2014.


Two key virulence factors of Helicobacter pylori are the secreted virulent proteins of vacuolating toxin A (VacA) and cytotoxin associated protein A (CagA) which lead to damages of gastric epithelial cells. We previously identified that the cyanidin 3-O-glucoside (C3G) inhibits the secretion of both VacA and CagA. In the current report, we show that C3G inhibits VacA secretion in a dose-dependent manner by inhibiting secretion system subunit protein A (SecA) synthesis. As SecA is involved in translocation of bacterial proteins, we predicted that inhibition of the SecA pathway by C3G should decrease H. pylori-induced cell death. To test this hypothesis, the human gastric cell line KATO III cells were co-cultured with H. pylori 60190 (VacA(+)/CagA(+)) and C3G. We found that C3G treatment caused a decrease in activation of the pro-apoptotic proteins caspase-3/-8 in H. pylori-infected cells leading to a decrease in cell death. Our data suggest that consumption of foods containing anthocyanin may be beneficial in reducing cell damage due to H. pylori infection.

Keywords: H. pylori; VacA secretion; cyanidin 3-O-glucoside.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / antagonists & inhibitors
  • Anthocyanins / administration & dosage*
  • Bacterial Proteins / antagonists & inhibitors
  • Bacterial Proteins / metabolism*
  • Cell Death / drug effects
  • Cell Line
  • Epithelial Cells / microbiology
  • Gastric Mucosa / drug effects*
  • Glucosides / administration & dosage*
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / genetics
  • Humans
  • Membrane Transport Proteins
  • SEC Translocation Channels
  • SecA Proteins
  • Signal Transduction / drug effects


  • Anthocyanins
  • Bacterial Proteins
  • Glucosides
  • Membrane Transport Proteins
  • SEC Translocation Channels
  • VacA protein, Helicobacter pylori
  • cyanidin-3-O-beta-glucopyranoside
  • Adenosine Triphosphatases
  • SecA Proteins