Polyphenols from blueberries modulate inflammation cytokines in LPS-induced RAW264.7 macrophages

Int J Biol Macromol. 2014 Aug;69:382-7. doi: 10.1016/j.ijbiomac.2014.05.071. Epub 2014 Jun 3.


Polyphenols including 3-glucoside/arabinoside/galactoside-based polymers of delphinidins, petunidins, peonidins, malvidins and cyanidins are one type of biological macromolecules, which are extraordinarily rich in blueberries. Anti-inflammatory activity of blueberry polyphenols (BPPs) was investigated by using lipopolysaccharide (LPS) induced RAW264.7 macrophages. The results showed that BPPs suppressed the gene expression of IL-1β (interleukin-1β), IL-6 and IL-12p35. The inhibition effect on IL-1β and IL-6 mRNA was most obvious at the concentration of 10-200μg/mL BPPs. But the inhibition effect on IL-12p35 mRNA was increased with the increasing concentration of BPPs. When fixed at 100μg/mL BPPs, the most significant inhibition on IL-1β, IL-6 and IL-12p35 mRNA expression was detected at 12-48h. In conclusion, BPPs exhibit anti-inflammation activity by mediating and modulating the balances in pro-inflammatory cytokines of IL-1β, IL-6, and IL-12.

Keywords: Anti-inflammation; Blueberries; Polyphenols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Blueberry Plants / chemistry*
  • Cell Line
  • Cytokines / genetics*
  • Gene Expression Regulation / drug effects*
  • Inflammation / genetics
  • Lipopolysaccharides / pharmacology*
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Mice
  • Polyphenols / pharmacology*


  • Anti-Inflammatory Agents
  • Cytokines
  • Lipopolysaccharides
  • Polyphenols